苏州阿尔法生物实验器材有限公司

细胞名称:u251细胞株-人胶质瘤细胞(STR鉴定正确)

细胞别称: U-251 MG; U-251-MG; U-251_MG; U251-MG; U251MG; U-251; U251; U251n; U251N; 251 MG; 251MG

细胞简称:u251细胞株

产品货号:TCH-C366

规格: 1×10⁶cells/ T25 培养瓶

价格:1000元 (干冰运输需另加200元干冰费)

u251细胞株-人胶质瘤细胞

细胞名称:u251细胞株-人胶质瘤细胞(STR鉴定正确)

细胞别称: U-251 MG; U-251-MG; U-251_MG; U251-MG; U251MG; U-251; U251; U251n; U251N; 251 MG; 251MG

细胞简称:u251细胞株

产品货号:TCH-C366

规格: 1×10⁶cells/ T25 培养瓶

价格:1000元 (干冰运输需另加200元干冰费)

种属来源:人

组织来源:神经胶质

特征:神经胶质细胞瘤

细胞形态:成纤维细胞样

生长特性:贴壁生长

培养体系:DMEM-H+10%FBS+1%P/S

u251细胞株-人胶质瘤细胞 配套培养基货号: TCH-G366

传代比例:1:3-1:6,每2-3天换液一次

传代周期:48-72 h

培养条件:气相:95%空气+5%CO2,温度:37℃

冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测:细菌、真菌、支原体检测均为阴性

u251细胞株-人胶质瘤细胞 分离于胶质母细胞瘤组织。 

U251说明书


参考文献:

"Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.

A map of mobile DNA insertions in the NCI-60 human cancer cell panel.

Mob. DNA 7:20-20(2016)

Allen M., Bjerke M., Edlund H., Nelander S., Westermark B.

Origin of the U87MG glioma cell line: good news and bad news.

Sci. Transl. Med. 8:354re3-354re3(2016)

Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.

Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.

Sci. Data 3:160052-160052(2016)"