Jurkat细胞
细胞名称： 人T淋巴细胞白血*细胞（STR鉴定正确）
细胞简称：Jurkat细胞
细胞别称：JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1
货号：TCH-C225
规格： 1×10⁶cells/T25 培养瓶
价格：1500元 (干冰运输需另加200元干冰费)
种属来源：人
组织来源：外周血
特征：急性T淋巴细胞白血*
细胞形态：淋巴母细胞样
生长特性：悬浮生长
培养体系：RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
Jurkat细胞 配套培养基货号：TCH-G225
传代比例：1:3-1:6，每2-3天换液一次
传代周期：24-48 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
该细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。生长方式为悬浮生长，生长速度较快，使用1640培养液培养。显微镜下观察，正常细胞状态为圆润透亮，似鱼卵。
Jurkat细胞株来源于一个14岁男孩的外周血；经弗波酯和外源凝集素或抗T3单克隆抗体中的一种诱导后可产生大量IL-2(IL-2的产生需两种类型的诱导剂)；表达T细胞受体、CD3。
苏州阿尔法生物实验器材有限公司除了提供细胞系购买，细胞株购买，pc12细胞株，肺癌细胞株，Jurkat细胞，血管内皮细胞株，肝癌细胞株，肿瘤细胞株，胃癌细胞株，hela细胞株，胰岛细胞株，s180细胞株，hela细胞株，动物细胞，干细胞外，还提供MEM培养基,DMEM,F12培养基,RPMI培养基等基础培养基，干细胞培养基,培养基添加剂,间充质干细胞培养基 ,完全培养基,低血清培养基,胎牛血清,鸡血清等细胞培养试剂。
参考文献：
"Weiss A, et al. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. 133: 123-128, 1984. PubMed: 6327821
Gillis S, Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J. Exp. Med. 152: 1709-1719, 1980. PubMed: 6778951
Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed: 9284127
Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194
Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663
Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842
Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984
Kung SH, Medveczky PG. Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J. Virol. 70: 1738-1744, 1996. PubMed: 8627695
Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082"
