苏州阿尔法生物实验器材有限公司

细胞名称: 人T淋巴细胞白血病细胞(STR鉴定正确)

细胞简称:Hut78细胞

细胞别名: Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78;HUT78; NCI-H78

货号:TCH-C218

规格:1×10⁶cells/ T25 培养瓶

Hut78细胞价格:1400元 (干冰运输需另加200元干冰费) 

Hut78细胞培养基 货号:TCH-G218

 

Hut78细胞


细胞名称: 人T淋巴细胞白血病细胞(STR鉴定正确)

细胞简称Hut78细胞

细胞别名: Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78;

HUT78; NCI-H78

货号:TCH-C218

规格:1×10⁶cells/ T25 培养瓶

价格:1400元 (干冰运输需另加200元干冰费)  

种属来源:人

组织来源:皮肤

特征:塞扎里综合症

细胞形态:淋巴母细胞

生长特性:悬浮生长

培养体系:IMDM+20%FBS+1%P/S

细胞配套培养基 货号:TCH-G218

传代比例:1:3-1:6,每2-3天换液一次

传代周期:48-72 h

培养条件:气相:95%空气+5%CO2,温度:37℃

冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测:细菌、真菌、支原体检测均为阴性

Hut78

NCI-H78 细胞 可产生IL-2,TNF-α;表达IL-2受体


参考文献:

Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346


Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177


Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013


Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674


O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285


Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991


Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128


Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082


Hut 78 was derived from peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.

Cancer Cell 31:225-239(2017)


苏州阿尔法生物实验器材有限公司创立于2008年,坐落于苏州工业园区生物纳米园,深耕生命科学实验室配套领域18年,是苏州本土专业一站式生物实验仪器、耗材、科研试剂综合服务商,长期为全国高校科研院所、生物医药企业、第三方检测中心、医疗机构实验室提供全流程配套解决方案。
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