细胞名称:huh7细胞 (STR鉴定正确)
细胞别称: HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39;
货号:TCH-C217
规格: 1X10^6
目录价格:1500元 (干冰运输需另加200元干冰费)
huh7细胞培养基货号:TCH-G217
huh7细胞
细胞名称:huh7细胞 (STR鉴定正确)
细胞别称: HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39; Japanese Tissue Culture-39
货号:TCH-C217
规格: 1X10^6
价格:1500元 (干冰运输需另加200元干冰费)
种属来源:人
组织来源:肝脏
特征:肝癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
huh7细胞培养基货号:TCH-G217
传代比例:1:2-1:3,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
huh-7细胞 产甲胎蛋白,胰酶α抗体,血浆铜蓝蛋白,纤维蛋白原,纤维粘连蛋白等。
参考文献:
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.
HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Hum. Cell 31:261-267(2018)
Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.
Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.
Drug Metab. Pharmacokinet. 33:133-140(2018)