苏州阿尔法生物实验器材有限公司

CHOK1细胞是CHO的一个亚克隆,为转化细胞系,   在工业生产上应用较多,  CHOK1细胞染色体分布频率是2n=22,系亚二倍体细胞。海星细胞库保存CHO-K1细胞株,编号为TCO-C005,被广泛地用于重组DNA蛋白的表达。

CHOK1细胞

1957年,PuckTT从成年中国仓鼠卵巢的活检组织建立了CHO细胞,CHOK1细胞是CHO的一个亚克隆。CHOK1细胞的生长需要脯氨酸。 

细胞名称:仓鼠卵巢细胞

细胞简称CHO-K1

细胞别称:CHO K1; CHOK1; CHO cell clone K1; GM15452

产品货号TCO-C005

规格: 1X10^6

种属来源仓鼠

组织来源卵巢

细胞形态上皮细胞样

生长特性贴壁生长

培养体系F12K+10%FBS+1%P/S

配套培养基货号:TCO-G005

传代比例:1:3-1:6,每2-3天换液一次

传代周期48-72 h

培养条件气相:95%空气+5%CO2,温度:37℃

冻存条件60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测细菌、真菌、支原体检测均为阴性

  CHOK1细胞为转化细胞系,   在工业生产上应用较多,  CHOK1细胞 染色体分布频率是2n=22,系亚二倍体细胞。海星细胞库保存CHO-K1细胞株,编号为TCO-C005,被广泛地用于重组DNA蛋白的表达。

CHOK1细胞


参考文献:

Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821


Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058


Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736


Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403


Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351


Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752


Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365