b16f10细胞-小鼠皮肤黑色素瘤细胞

b16f10细胞是B16-F0的亚系.

细胞名称： 小鼠皮肤黑色素瘤细胞

简称：b16f10细胞

细胞别名： B16/F10; B16 F10; B16F10; B16 melanoma F10

货号：TCM-C713

规格： 1X10^6

价格：140 0元 (干冰运输需另加200元干冰费)  

种属来源：小鼠

组织来源：皮肤

特征：黑色素瘤

细胞形态：上皮细胞样混有梭形细胞

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%P/S

b16f10细胞培养基货号：TCM-G713

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

b16f10细胞冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

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参考文献：

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Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034

Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927

Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183

Fidler IJ. Selection of successive tumour lines for metastasis. Nat. New Biol. 242: 148-149, 1973. PubMed: 4512654

Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77: 2757-2765, 1996. PubMed: 8922469