苏州阿尔法生物实验器材有限公司

细胞名称:L-929细胞-小鼠成纤维细胞

细胞别称: NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles's cells; Earle's L cells

规格: 1X10^6| T25培养瓶

价格:1500元 (干冰运输需另加200元干冰费)

L-929细胞-小鼠成纤维细胞 配套培养基货号:TCM-G749

L-929细胞-小鼠成纤维细胞

细胞名称:L-929细胞-小鼠成纤维细胞

细胞别称: NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); 

Clone 929; Earles's cells; Earle's L cells

规格: 1X10^6| T25培养瓶

价格:1500元 (干冰运输需另加200元干冰费)

种属来源:小鼠

组织来源:皮下结缔组织

细胞形态:成纤维细胞样

生长特性:贴壁生长

培养体系:MEM+10%FBS+1%P/S

L-929细胞-小鼠成纤维细胞 配套培养基货号:TCM-G749

传代比例:1:3-1:6,每2-3天换液一次

传代周期:48-72 h

培养条件:气相:95%空气+5%CO2,温度:37℃

冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测:细菌、真菌、支原体检测均为阴性

L-929

      1948年三月建立了NCTCclone929(小鼠结缔组织),细胞系L的克隆。细胞系L是较早建立的连续培养细胞系之一,而clone929是较早的克隆株。从一只100日龄的雄性C3H/An小鼠的正常皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L。第95代的细胞系L使用毛细管法分离单细胞建立了clone929。


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